Journal: International Journal of Molecular Sciences
Article Title: Ischemic Rescue Potential of Conditioned Medium Derived from Skeletal Muscle Cells-Seeded Electrospun Fiber-Coated Human Amniotic Membrane Scaffolds
doi: 10.3390/ijms252111697
Figure Lengend Snippet: The effects of CM on HCM subjected to H/R-induced injury according to the XIAP expression profile. Immunofluorescence images of HCM stained with XIAP antibody (red) and DAPI (blue) after being treated with different CM following H/R-induced injury (10× magnification; 200 µm scale bar; n = 3). The bar graph represents the MFI measurement for XIAP in H/R-injured HCM after ischemic rescue with CM. Treatment with different EF–HAM CM did not upregulate the expression of XIAP injured HCM. The values are represented as mean ± SEM ( n = 3; * p < 0.05 or ** p < 0.01, compared with Normoxic control; one-way ANOVA).
Article Snippet: The HCMs were incubated with mouse monoclonal anti-human XIAP (A-7) antibody (1:50 dilution; Santa Cruz Biotechnology, Dallas, TX, USA), followed by immunolabeling by goat anti-mouse Alexa Fluor ® 594 secondary antibody (1:300 dilution) and counterstaining with DAPI.
Techniques: Expressing, Immunofluorescence, Staining, Control